Acclaim PolarAdvantage Reversed-Phase LC Column
Acclaim® PolarAdvantage (PA) columns feature a patented bonding chemistry that incorporates a polar sulfonamide group near the surface of the silica particle. This unique chemistry provides good hydrolytic stability and allows a single column to resolve a wide variety of polar and nonpolar analytes. The Acclaim PA is compatible with* aqueous mobile phases and supports LC/MS analysis.
Conventional C18 phases are not compatible with highly aqueous mobile phases, due to dewetting. The Acclaim PA column by design has a mildly hydrophilic surface, which remains in contact with aqueous-only mobile phases, negating the problems of dewetting common with conventional reversed-phase columns.
Acclaim PA columns are available both in 3 and 5 μm particle sizes and 4.6, 3.0, and 2.1 mm diameters, with an average pore diameter of 120 Å. Acclaim PA is also available in 2.2µm RSLC format (see Acclaim Rapid Separation LC (RSLC) Columns section for more details). Acclaim PA may be used for LC/MS applications.
The Acclaim PA column can be used in a wide range of applications in pharmaceutical, environmental, food testing, and product-quality testing. These columns also give efficient separations with good peak shapes for compounds of life science (nucleic acid and bases) and pharmaceutical interest (sulfonamides) using highly aqueous mobile phases.
Standard particle sizes are 3 and 5 μm. Available in 2.1, 3.0, and 4.6 mm i.d. Lengths are 50, 100, and 150 mm. Capillary formats are available in 75 and 300 μm, 1.0 mm, and custom diameters, and in 50, 150, or 250 mm lengths. Micro precolumn and nano precolumn cartridges are available in several formats. Guard columns in both 2.0 and 4.3 mm sizes packed with 5 μm particles are recommended. Columns for fast LC are listed in the RSLC section.